Isaac Scientific Publishing

Journal of Advances in Molecular Biology

Homology Modeling and Functional Annotation of Heat Shock Protein (HSP) Gene from Agave Americana

Download PDF (401.8 KB) PP. 113 - 119 Pub. Date: September 1, 2017

DOI: 10.22606/jamb.2017.12004


  • Anicet Batcho*
    Plant Genomic Lab, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore
  • Bilal Sarwa
    Plant Genomic Lab, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore
  • Bushra Rachid
    Plant Genomic Lab, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore


Identifying active domain in a protein sequence is one of the most important functions in molecular biology. This is usually studied with three-dimensional (3D) structure of the active sites protein using Bioinformatics tools. In the absence of an experimentally determined structure, homology modeling can sometimes supply a useful 3D model for protein that is linked to at least one known protein structure. In this work, we have evaluated the sequence information of Heat Shock Protein (HSP) gene retrieved from Agave americana. Homology modeling, functional annotation of A. americana heat shock protein (HSP) sequence. Based on homology modeling, 3D structure of the gene was constructed. Validation tests were used to calculate and determine the reliability of the structure. In this study, details structure of HSP led to develop a better understanding of the HSP’s role in different abiotic stress response pathways.


Functional annotation, bioinformatics tools, HSP gene, agave americana


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