Isaac Scientific Publishing


Electromagnetic Field Changes Emotional-Motivated Behavior in Genetically Epilepsy-Prone Rats

Download PDF (204.5 KB) PP. 13 - 18 Pub. Date: November 3, 2018



  • Nato Bukia*
    Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
  • Giorgi Kekelia
    Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
  • Marina Butskhrikidze
    Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
  • Lamara Machavariani
    Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
  • Militsa Svanidze
    Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia


In recent years, electro-magnetic field (EMF) exposure is tested as a tool for diagnosis and treatment of the wide range of neurological and psychological disorders (including epilepsy). In the present study, we examined the effects of acoustic range EMF on emotional-motivated behavior in genetically epilepsy-prone rats (GEPRs) of Krushinsky-Molodkina strain in Open field test. Methods. For this reason, generator of EMF with original coil design was used. The optimal parameters of EMF, which partially or fully inhibited the behavioral manifestation of seizure were established during pilot experiments. These were 10-15 kHz, 1-1,5 milliTesla, duration 20 min. Before and after EMF exposure in open field test, parameters of emotional-motivated behavior - entering the center, numbers of crossed squares, head raise, vertical stands, the frequency and duration of grooming, number of fecal boluses and urination were registered; Data reliability was assessed by using parametric and non-parametric techniques, with the use of one- and two-way layout of factorial analysis. Results and Discussion. On the background of a 5-days exposure of electric-magnetic field (EMF) the number of crossed squares (from 100±8 to 120±12, p≤0.01), hole reflex (from 17±2 to 24±3, p≤0.01) and head lifts (from 11 ±2 to 14±4, p≤0.05) were increased. The number of vertical stands (16±2 to 12±1 P≤ 0.05), duration (from 21 ±2 to 15±4, p≤0.05) and the number of grooming episodes (from 75 ±7 to 45±6 P≤0.01), as well as the number of fecal boluses and the frequency of urination were decreased. These findings suggest that acoustic range magnetic field decreases anxiety degree and consequently, enhances locomotor and exploratory activity of the GEPRs. A 5-days exposure EMF to inbred white rats did not change significantly locomotion/exploratory activity in open field test. Conclusion. Acoustic range EMF can be applied for suppression of behavioral manifestation of seizure. These data support a potential role of EMF exposure in changing emotion regulation of anxiety, but the mechanisms of such influence are not known. Proposed biological mechanisms in anti-seizure and anxiolytic effects of EMF exposure in GEPRs might include normalization of neuroendocrine, neurotransmitter, and/or neurotrophic factors.


Electromagnetic field; seizure; rats; general behavior


[1] Adey W.R., Collective properties of cell membranes. In: Norden B, Ramel K, eds., Interaction Mechanisms of Low-Level Electromagnetic Fields in Living Systems. Oxford: Oxford University Press, 47-77, 1992.

[2] Aldridge JW, Berridge KC, Rosen AR. Basal ganglia neural mechanisms of natural movement sequences. Can J Physiol Pharmacol 82:732–739. 2004.

[3] Berridge KC, Aldridge JW, Houchard KR, Zhuang X. Sequential super-stereotypy of an instinctive fixed action pattern in hyperdopaminergic mutant mice: a model of obsessive compulsive disorder and Tourette’s. BMC Biol 3:1–16, 2005.

[4] Cohen OS, Orlev Y, Yahalom G, Amiaz R, Nitsan Z, Ephraty L, Rigbi A, Shabat C, Zangen A, Hassin-Baer S. Repetitive deep transcranial magnetic stimulation for motor symptoms in Parkinson's disease: A feasibility study. Clin Neurol Neurosurg. 140:73-78. 2016.

[5] David Eilam. Open-field behavior withstands drastic changes in arena size. Behav. Brain Res. 142, 53–62, 2003.

[6] Garcia-Cairasco N A critical review on the participation of inferior colliculus in acoustic-motor and acousticlimbic networks involved in the expression of acute and kindled audiogenic seizures Hear Res. 168(1-2):208-222. 2002.

[7] He LH, Shi HM, Liu TT, Xu YC, Ye KP, Wang S. Effects of extremely low frequency magnetic field on anxiety level and spatial memory of adult rats,124(20):3362-3366, 2011.

[8] K. van Rijckevorsel Cognitive problems related to epilepsy syndromes, especially malignant epilepsies seizure, 2006.

[9] Kim JW, Bae KY, Kim SW, Kang HJ, Shin IS, Yoon JS, Kim JM. Treatment-Resistant depression entering remission following a seizure during the course of repetitive transcranial magnetic stimulation. Psychiatry Investig. 13(4):468-471, 2016.

[10] Marescaux C, Vergnes M, Kiesmann M, Depaulis A, Micheletti G, Warter JM. Kindling of audiogenic seizures in Wistar rats: an EEG study. Exp Neurol. 97(1):160-168. 1987.

[11] Melissa M.Carballosa-Gonzaleab,Luis, J.Mu?nozc, Tomás López-Alburquerquea, José Manuel Pardal-Fernándezd, Eduardo Navae, Carlos de Cabo, Consuelo Sanchoa, Dolores E.López EEG Characterizatio of audiogenic Seizures in the hamster strain GASH. Sal. Epilepsy research 106, 318-325, 2013.

[12] Mesquita RC, Faseyitan OK, Turkeltaub PE, Buckley EM, Thomas A, Kim MN, Durduran T, Greenberg JH, Detre JA, Yodh AG, Hamilton RH. Blood flow and oxygenation changes due to low-frequency repetitive transcranial magnetic stimulation of the cerebral cortex. J Biomed Opt.,18(6), 2013.

[13] Okaichi Y, Amano S, Ihara N, Hayase Y, Tazumi T, Okaichi H. Open-field behaviors and water-maze learning in the F substrain of Ihara epileptic rats. Epilepsia. 47(1):55-63. 2006.

[14] Pereira LS, Müller VT, da Mota Gomes M, Rotenberg A, Fregni F. Safety of repetitive transcranial magnetic stimulation in patients with epilepsy: A systematic review. Epilepsy Behav.,57 (Pt A):167-76, 2016.

[15] Roeling TA, Veening JG, Peters JP, Vermelis ME, Nieuwenhuys R. Efferent connections of the hypothalamic "grooming area" in the rat. Neuroscience 56:199–225, 1993.

[16] Semiokhina AF, Fedotova IB, Poletaeva II. [Rats of Krushinsky-Molodkina strain: studies of audiogenic epilepsy, vascular pathology, and behavior]. Zh Vyssh Nerv Deiat, Im I. P. Pavlova. 56(3):298-316, 2006.

[17] Shin EJ, Jeong JH, Kim HJ, Jang CG, Yamada K, Nabeshima T, Kim HC Exposure to extremely low frequency magnetic fields enhances locomotor activity via activation of dopamine D1-like receptors in mice., J Pharmacol Sci.,105(4):367-371, 2007.

[18] Thompson PJ, Conn H, Baxendale SA, Donnachie E, McGrath K, Geraldi C, Duncan JS. Optimizing memory function in temporal lobe epilepsy. Seizure. 38:68-74. 2016.

[19] Vataev SI, Malgina NA, Oganesian GA, Sechenova IM. Inferior colliculus stimulation effects in KrushinskiIMolodkina strain rats. Ross. Fiziol. 100(6):699-709, 2014.