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Ecology and Sustainable Development
ESD > Volume 2, Number 2, May 2019

Fatty Acid Composition in Cultured Amphiascoides neglectus (Copepoda: Harpacticoida)

Download PDF  (331.8 KB)PP. 25-32,  Pub. Date:May 30, 2019
DOI: 10.22606/esd.2019.22001

Author(s)
Zaleha Kassim, Amir Safwan Hamzah, Hidayah Atirah Hashim, Hazwani Hanim Hasnan
Affiliation(s)
Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
Abstract
This study investigates the effect of salinity on the fatty acid profile of a harpacticoid copepod Amphiascoides neglectus exposed to constant laboratory lighting and temperature (25-26oC) at pH7 for 40 days. The results were compared with those cultured in the common open hatchery environment which is almost the same as in the natural ecosystem. Different fatty acid levels were determined using Gas Chromatography and Mass Spectrometry (GC-MS). Results showed that there were no significant differences (p>0.05) in fatty acid content for both 20ppt and 30ppt treatment but the percentage of EPA was higher in 20ppt. Harpacticoid copepods in the hatchery showed significantly (p<0.05) more fatty acids in 30ppt than 20ppt. EPA was also higher in harpacticoids cultured under 30ppt. Manipulation of salinity and temperature in combination of light factor for copepod rearing system could improve the deposition of fatty acids in their body.
Keywords
Harpacticoid culture, fatty acid, EPA, culture environment
References
  • [1]  J.G. Støttrup and N.H. Norsker, “Production and use of copepods in marine fish larviculture,” Aquaculture, vol. 155, no. 4, pp. 231-247,1997.
  • [2]  A.D. McKinnon, S.Duggan, P.D.Nochols, M.A. Rimmer, G. Semmens and B. Robino, “The potential of tropical paracalanid copepods as live feeds in aquaculture”, Aquaculture, vol. 223, pp. 8-106, 2003.
  • [3]  K. Zaleha, A. John, H. Asgnari, Al-Aama, and M.A. Fuad, “Fatty acid profiling of benthic harpacticoid (Pararobertsonia sp.) exposed to environmental stresses,” Malaysia Applied Biology, vol.43, no. 1, pp. 31–40, 2014.
  • [4]  M. Dias, A. Silva, H. Cabral and C. Vinagre, “Diet of marine fish larvae and juveniles that use rocky intertidal pools at the Portuguese Coast,” Journal of Applied Ichthyology, vol. 30, no. 5, pp. 970-977. 2014.
  • [5]  T. Mascart, G. Lepoint, S. Deschoemaeker, M. Binard, F. Remy and M. De Troch, “Seasonal variability of meiofauna, especially harpacticoid copepods,in Posidonia oceanica macrophytodetritus accumulations,” Journal of Sea Research, vol. 95, pp.149-160, 2015.
  • [6]  J.M. Gee, “An Ecological and Economic Review of Meiofauna as Food for Fish,” Zoological Journal of the Linnean Society, vol.96, pp. 243-261, 1989.
  • [7]  S.Shimode and Y. Shirayama, “Diel Vertical Migration and Life Strategies of Two Phytal-dwelling Harpacticoids, Ambunguipes rufocincta and Eudactylopds spectabilis,” Plankton and Benthos Research, vol.1, no. 1, pp. 42-53, 2006.
  • [8]  Zaleha Kassim, Muhammad Solleh Majid, Nur Yahdiyani Mat Alwi, Hazwani Hanim Hasnan. Diet Preferences of Small Fishes at Setiu Lagoon, Malaysia. Ecology and Sustainable Development, vol. 1, no.1, pp. 9-17. 2018.
  • [9]  C.J. Cutts, “Culture of harpacticoid copepods: Potential as live feed for rearing marine fish,” Advances in Marine Biology, vol. 44, pp. 295-316, 2003.
  • [10]  L.C.M .Lima, D.M.A.F. Navarro and L. P. Souza-Santos, “Effect of diet on the fatty acid composition of the copepod Tisbe Biminiensis,” Journal of Crustacean Biology, vol. 33, no.3, pp. 372-381, 2013.
  • [11]  E. Werbrouck, D.V. Gansbeke, A. Vanreusel and M.D. Troch, “Temperature Affects the Use of Storage Fatty Acids as Energy Source in a Benthic Copepod (Platychelipus littoralis, Harpacticoida),” PLoS ONE, vol.11, no.3, pp. 1-16, 2016.
  • [12]  S.H. Lee, M.C. Lee, J. Puthumana, J.C. Park, S. Kang, J. Han, K.H. Shin, H.G. Park, A.S. Om and J.S. Lee, “Effects of temperature on growth and fatty acid synthesis in the cyclopoid copepod Paracyclopina nana,” Fisheries Science, vol.83, no.5, pp. 725-734, 2017.
  • [13]  J. Garzke, T. Hansen, S.M.H. Ismar and U. Sommer, “Combined Effects of Ocean Warming and Acidification on Copepod Abundance, Body Size and Fatty Acid Content” PLoS ONE, vol. 11, no. 5, pp.1-22, 2016.
  • [14]  C. Ladhar, H. Ayadi, F. Denis, E.Tastard and I. Sellami, “The effect of environmental factors on the fatty acid composition of copepods and Artemia in the Sfax solar saltern (Tunisia)” Biochemical Systematics and Ecology, vol. 56, pp. 237–245, 2014.
  • [15]  S. Bollmohr, R. Schulz, and T. Hahn, “Interactive effect of salinity decrease, salinity adaptation, and chlorpyfiros exposure on an estuarine harpacticoid copepod, Mesochra parva, in South Africa,” Ecotoxicology and Environmental Safety, vol. 72, no. 3, pp.756-64, 2009.
  • [16]  D.A.Nanton and J.D. Castell, “The effects of dietary fatty acids on the fatty acid composition of the harpacticoid copepod, Tisbe sp., for use as a live food for marine fish larvae,” Aquaculture, vol. 295, no.1-2, pp.120-125, 1998.
  • [17]  M. Püttmann, H. Krug, E.V. Ochsentein and R. Kattermann, “Fast HPLC determination of serum free fatty acids in the picomole range” Clinical Chemistry, vol.39, pp. 825-832, 1993.
  • [18]  K. Ichihara and Y. Fukubayashi, “Preparation of fatty acid methyl esters for gas-liquid chromatography,” Journal of Lipid Research, vol. 51, no.3, pp.635-640, 2010.
  • [19]  R.M. Damgaard and J. Davenport, “Salinity tolerance, salinity preference and temperature tolerance in the high-shore harpacticoid copepod Tigriopus brevicornis,” Marine Biology, vol.118, no.3, pp. 443-449, 1994.
  • [20]  K. Zaleha and I. Busra, “Culture of harpacticoid copepods: understanding the reproduction and effect of environmental factors,” in Aquaculture. InTech, 2012, pp. 343-360.
  • [21]  B. Sun and J.W. Fleeger, “Sustainable mass culture of Amphiascoides atopus a marine harpacticoid copepod in a recirculating system,” Aquaculture, vol.136, pp. 313–321, 1995.
  • [22]  H. Matias-Peralta, F.M. Yusoff, M. Shariff and A. Arshad, “Effects of some environmental parameters on the reproduction and development of a tropical marine harpacticoid copepod Nitocra affinis f. californica Lang,” Marine Pollution Bulletin, vol.51, pp.722–728, 2005.
  • [23]  A. Ventosa, A. Oren and Y. Ma, Halophiles and Hypersaline Environments: Current Research and Future Trends. Springer. 2011.
  • [24]  M.T. Arts, R.G. Ackman and B.J. Holub, “Essential fatty acids in aquatic ecosystems : A crucial link between diet and human health and evolution,” Canadian Journal of Fishery and Aquaculture Science, vol. 58, pp. 122– 137, 2001.
  • [25]  C. Tapia, L. Parra, A. Mutis, G. Gajardo and A. Quiroz, “Fatty acid methyl esters extracted from the cuticular surface of Artemia franciscana (Kellogs, 1906) (Crustacea: Anostraca) increase the swim speed of conspecific males,” Gayana, vol. 80, no.2, pp. 154-160, 2016.
  • [26]  S. M. Renaud and D.L. Parry, “Microalgae for use in tropical aquaculture II: Effect of salinity on growth, gross chemical composition and fatty acid composition of three species of marine microalgae,” Journal of Applied Phycology, vol. 6, no. 3, pp. 347-356, 1994.
  • [27]  L.K. Chin and K. Zaleha, “Effect of photoperiod on the cellular fatty acid composition of three tropical marine microalgae,” Malaysian Applied Biology, vol. 42, no. 1, pp. 41-49, 2013.
  • [28]  S. Kaartvedt, “Photoperiod may constrain the effect of global warming in artic marine system,” Journal of Plankton Research, vol. 30, no. 11, pp. 1203-1206, 2008.
  • [29]  G. Drillet, S. Frouël, M.H. Sichlau, P.M. Jepson, J.K. Højgaard, A.K. Joarder and B.W. Hansen, “Status and recommendations on marine copepod cultivation for use as live feed,” Aquaculture, vol. 315, pp. 155–166, 2011.
  • [30]  N. Nogueira, B. Sumares, C.A.P. Andrade and A. Afonso, “The effects of temperature and photoperiod on egg hatching success, egg production and population growth of the calanoid, Acartia grani (Calanoida: Acartiidae)” Aquaculture Research, 49(1): 93-103, 2018.
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