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Annals of Advanced Agricultural Sciences
AS > Volume 4, Number 2, May 2020

Phase Change Related microRNA Profiles in the Plant Regeneration Process of Avocado through Shoot-tip Culture

Download PDF  (374.4 KB)PP. 9-17,  Pub. Date:March 25, 2020
DOI: 10.22606/as.2020.42001

Author(s)
Jayeni Hiti-Bandaralage, Alice Hayward, Chris O’Brien, Umair Ahsan, Madeline Gleeson, Yuxin Xue, Neena Mitter
Affiliation(s)
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, Australia
Abstract
MicroRNAs are a group of non-coding, highly conserved, small RNAs of 21-22 nucleotides found in both animals and plants. They regulate plant development by cleavage of complimentary mRNAs or translational inhibition. Avocado is a high value crop, which can be benefited by tissue culture for propagation. It has been hypothesised that tissue culture process restores juvenility in physiologically mature explants. The current study examined this by profiling two microRNAs, miR156 and miR172, involved in juvenile-to-adult phase change. The study revealed that, both miR156 and miR172 are expressed at similar levels in cultured avocado shoot meristems. MicroRNAs levels were significantly increased during the shoot elongation stage. After the acclimatization, microRNAs relative expression levels were re-established to the level of explant material used. This may suggest that the maturity state of plants, as determined by miR156 and miR172 profile, remains unchanged in propagated plants rejecting the hypothesis.
Keywords
avocado, phase change, microRNA 156/172, micropropagation.
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